Identification and evaluation index screening of low temperature toler-ance of bitter gourd seedling of different genotypes
WANG Zisong
SHEN Wei
FANG Lijuan
YANG Jing
WANG Taotao
LI Rongfeng
Abstract：The effects of low temperature stress on physiology and biochemistry of Momordica charantia L.were studied to screen low-temperature-tolerant materials and evaluation indicators in 41 inbred seedlings.The results showed that the variation coefficient of 12 physiological and biochemical indicators among the 41 bitter gourd inbred lines were 5％-150％,which could be transformed into 5 independent comprehensive indicators.The cumulative contribution rate of the main factors reached 74.22％.Correlation analysis indicated that Fv/Fm was significantly negatively correlated with MDA content and soluble sugar content.The 41 different genotypes of bitter gourd inbred lines could be classified into 5 categories based on the strength of low-temperature tolerance evaluated by the membership function method.Six inbred lines with relatively strong low-temperature tolerance were screened out,and Pro content,SOD activity,Fv/Fm,MDA con-tent and Ci were established as the evaluation indicators of low-temperature tolerance,which provides a relatively effi-cient and applicable method for the efficient and early-maturing breeding of bitter gourd and the identification of low-tem-perature tolerance materials.
Keywords：Bitter gourdLow temperature stressPrincipal factor analysisMembership functionEvaluation index
Publication Date：2025-11-05
Online Publishing Date：2025-12-04(First online date of this platform, not the publication date of the document)
Pages：8( 36-43 )