Screening and identification of suitable host for potato virus A

ZHANG Wei; WEI Xuyan; FAN Guoquan; GAO Yanling; SUN Xuhong; BAI Yanju

doi:10.16861/j.cnki.zggc.2025.0046

Abstract：Potato virus A(PVA)is one of the significant viruses infecting potato,belonging to the Potyvirus genus,and can reduce global potato yields by 30％-40％.Understanding the identification,propagation hosts,and biological charac-teristics of PVA is crucial for studying its pathogenic mechanisms and developing effective control strategies.In this study,PVA was inoculated into 18 indicator plants using the friction inoculation method.Through combined observation of biological symptoms and RT-PCR detection,systemically infected propagation hosts and locally infected diagnostic hosts were screened.TaqMan real-time quantitative PCR(TaqMan RT-qPCR)was employed to track the disease progres-sion in propagation hosts,studying the changes in viral content and biological manifestations of virus accumulation rate in different hosts.By integrating host size,total leaf area,and viral accumulation dynamics,the optimal propagation host for PVA was identified.The results showed that Physalis floridana developed localized necrotic spots on inoculated leaves,making it a suitable diagnostic host for PVA.Nicondra physaloides,Nicotinana rebneyi,Nicotiana rustica,Nicotiana tabacum,and Nicotiana tasacumca sanctum exhibited systemic infections and were identified as propagation hosts.Con-sidering plant size,total leaf area,and viral accumulation rates,Nicotiana rustica emerged as the most suitable propaga-tion host for PVA.This study provides basic datas for further research and scientific prevention on PVA.