Process optimization of sugar production by lignocellulose enzymatic hy-drolysis in spent Lentinus edodes substrate and its composition analysis
LIU Lina
LI Shunfeng
CUI Guomei
WEI Shuxin
WANG Anjian
YANG Chao
Abstract：In order to enhance the utilization efficiency of spent Lentinus edodes substrate,this study used it as the main raw material to produce sugars through lignocellulose enzymatic hydrolysis.Key process conditions were optimized using single-factor and orthogonal design experiments,,and changes in lignocellulose composition as well as infrared spectros-copy characteristics before and after enzymatic hydrolysis were compared.The results showed that the optimal conditions for sugar production from lignocellulose in spent Lentinus edodes substrate were as follws:Compound enzyme(cellulase/xylanase)ratio of 1∶1,compound enzyme addition of 3000 U·g-1,solid-liquid ratio of 1∶2,and enzymolysis time of 8 h.Under these conditions,the reducing sugar content reached 5.472％,which was 2.304 times higher than that of the raw materials.After enzymatic hydrolysis,the content of hemicellulose and cellulose decreased by 26.564％and 12.105％,respectively.Infrared spectroscopy analyais revealed that the characteristic absorption peaks of the lignocellulose functional groups remained unchangeed in position,but weakened in intensity after enzymatic hydrolysis,which was consistent with the degradation trends of the two component.In conclusion,enzymatic hydrolysis effectively increased the yield of reduc-ing sugar and promoted the degradation of lignocellulose in spent L.edodes substrate.This study provides a scientific basis for the high-value utilization of spent L.edodes substrate.
Keywords：Spent L.edodes substrateEnzymatic hydrolysisLignocelluloseInfrared spectroscopy
Publication Date：2025-10-05
Online Publishing Date：2025-11-05(First online date of this platform, not the publication date of the document)
Pages：7( 68-74 )