Preliminary study on the biocontrol mechanism of Paenibacillus poly-myxa YF against tomato gray mold
ZHANG Jianqiang
WEI Tao
LAI Xin
WANG Lihua
LIANG Jian
TANG Gong
Abstract：Tomato gray mold is a devastating fungal disease caused by Botrytis cinerea,which is one of the important fac-tors restricting tomato yield and quality.This study focused on Botrytis cinerea and its biocontrol bacteria were used as the research object,and explored the control effect and mechanism of the biocontrol bacteria.Plate confrontation assays showed that Paenibacillus polymyxa YF exhibited an inhibition rate of 41.04％against B.cinerea.Microscope observation revealed significant morphological abnormalities in the hyphae at the conony edges,characterized by thickened hyphae,in-creased branching,shortened internoded,and the presence of vesicle-like structures.Enzyme activity plate detection fur-ther showed that P.polymyxa YF could produce chitinase,β-1,3-glucanase and protease.When the fermentation broth was extracted with n-butanol and the extract was dissolved in methanol to 1 mg·mL-1 solution for plant confrontation assyas,the inhibition rate increased to 67.86％.In addtion,strain YF exhibited broad-spectrum inhibitory activity against eight common plant pathogens,with the lowest inhibition rate was 57.69％against Sclerotinia sclerotiorum and the highest of 68.57％against Rhizoctonia solani.In summary,P.polymyxa YF has significant antagonistic activity and antibacterial sub-stance secretion ability,providing a highly promising biocontrol strain for the biological control of tomato gray mold.
Keywords：TomatoBotrytis cinereaBiological controlPaenibacillus polymyxaControl mechanism
Publication Date：2025-10-05
Online Publishing Date：2025-11-05(First online date of this platform, not the publication date of the document)
Pages：7( 61-67 )