Identification and application of pumpkin molecular markers on root-stock with bloom-reducing from cucumber surface after grafting
PEI Yifan
ZHENG Xiu
BIE Zhilong
ZHOU Yuzhong
WANG Zhongquan
CHENG Jintao
Abstract：To develop molecular markers for Cucurbita maxima rootstocks related to their ability to remove cucumber fruit surface's bloom,evaluated 18 different C.maxima materials with varying bloom removal capabilities through graft-ing.The results showed that when cucumber was grafted onto rootstocks with strong bloom removal abilities,the silicon content in the stems,leaves,and fruits of the scion cucumber was significantly lower compared to those grafted onto root-stocks with weak bloom removal abilities.This indicates that the bloom removal capability of C.maxima is associated with its ability to absorb and transport silicon.Using the previously reported molecular marker CM1-CAPS based on SNP of the silicon transporter gene CmLSi1,along with the SNP-Sanger sequencing and Hi-Tom sequencing methods designed in this study,we identified and analyzed 57 C.maxima rootstocks with varying abilities to bloom removing.The results showed that all three identification methods achieved a 100％accuracy rate for selecting the bloom removing trait,indicat-ing that this molecular marker is suitable for breeding bloom removing rootstocks,which is of great significance to accel-erate the breeding of new varieties.
Keywords：Rootstock pumpkinCucumberBloom removalMolecular markerSilicon transporter protein
Publication Date：2025-10-05
Online Publishing Date：2025-11-05(First online date of this platform, not the publication date of the document)
Pages：9( 39-47 )