Screening and analysis of genes related to heat resistance in Lentinus edodes based on transcriptome sequencing
ZHAO Xinping
LIU Jinling
WANG Yufeng
LI Dongdong
DING Liang
WEN Yamei
LIU Qin
KONG Weili
Abstract：To explore key genes involved in Lentinula edodes(shiitake mushroom)response to heat stress,transcriptome sequencing analysis was performed using the heat-tolerant strain Xiang 931 and the heat-sensitive strain Nongke 1.Myce-lia were subjected to high-temperature treatment at 36℃,with normal temperature cultivation serving as the control(CK).The results showed that compared to the CK group,the mycelial growth rate of both Xiang 931 and Nongke 1 sig-nificantly decreased after high-temperature treatment.Strain-specific differentially expressed genes(DEGs)in response to high temperature were 740 for Xiang 931 and 702 for Nongke 1.KEGG and GO enrichment analysis of the DEGs re-vealed that genes from both strains were primarily and significantly enriched in metabolism-related functional pathways and processes,although the number of enriched genes differed between the two strains.Combined analysis based on KOG annotation and relevant databases indicated that laccase genes and HSP20 family genes exhibited significant expression differences between treatments,suggesting they are potential regulatory genes associated with heat tolerance in L.edodes.Six DEGs were randomly selected for qRT-PCR validation,and the results confirmed that the gene expression trends were consistent with the transcriptome sequencing data.This study provides a foundation for research into the molecular mecha-nisms of heat tolerance in L.edodes and the breeding of new heat-tolerant cultivars.
Keywords：Lentinus edodesTemperatureMycelial growthTranscriptomeDifferential gene expression
Publication Date：2025-10-05
Online Publishing Date：2025-11-05(First online date of this platform, not the publication date of the document)
Pages：11( 18-28 )