Research on in vitro culture and bulblet enlargement technologies of edi-ble and medicinal lily resources
TU Wenlong
LI Nan
YIN Jiaqi
ZHAO Jimin
MA Yanli
Abstract：This study systematically evaluated the effects of different disinfection methods and plant growth regulator(PGR)concentration ratios on the in vitro regeneration of five kinds of edible and medicinal lily(Dahua Juandan,Lan-zhou lily,Tiger lily,Longya lily,and Yixing lily)scale explants,and explored the mechanism of different concentration combinations of NAA,IBA,and SUC on the bulblet enlargement of Dahua Juandan using single-facter experiments and response surface methodology(RSM).The results showed that:(1)The optimal disinfection protocal for explants was 75％ethanol for 30 s followed by 0.1％HgCl2 for 12 min.(2)The optimal medium for adventitious bud induction:MS+1 mg·L-1 6BA+0.3 mg·L-1 NAA+30 g·L-1 sucrose(SUC)for Dahua Juandan and Lanzhou lily,MS+2 mg·L-1 6BA+0.1 mg·L-1 NAA+30 g·L-1 SUC for Tiger lily,MS+1 mg·L-1 6BA+0.2 mg·L-1 NAA+30 g·L-1 SUC for Longya lily,and MS+2 mg·L-1 6BA+0.2 mg·L-1 NAA+30 g·L-1 SUC for Yixing lily.(3)The optimal formula for subculture and prolifera-tion:Lanzhou lily,Tiger lily,and Yixing lily were MS+1 mg·L-1 6BA+0.2 mg·L-1 NAA+0.1 mg·L-1 KT+30 g·L-1 SUC,while Dahua Juandan and Longya lily were MS+0.5 mg·L-1 6BA+0.1 mg·L-1 NAA+0.1 mg·L-1 KT+30 g·L-1 SUC,and Longya lily is MS+1.5 mg·L-1 6BA+0.3 mg·L-1 NAA+0.5 mg·L-1 KT+30 g·L-1 SUC,respectively.(4)The best for-mula for rooting culture:MS+1 mg·L-1 IBA+0.5 mg·L-1 NAA+30 g·L-1 SUC for Dahua Juandan,MS+2 mg·L-1 IBA+0.5 mg·L-1 NAA+30 g·L-1 SUC for Lanzhou lily,MS+2 mg·L-1 IBA+0.1 mg·L-1 NAA+30 g·L-1 SUC for Tiger lily and Yixing lily,MS+3 mg·L-1 IBA+0.1 mg·L-1 NAA+30 g·L-1 SUC for Longya lily.(5)RSM optimization revealed that the best combination to promote the bulblet expansion of Dahua Juandan was 1.15 mg·L-1 NAA,2.14 mg·L-1 IBA,and 43.71 g·L-1 SUC.The predicted mass gain was 0.746 g,and the actual mass gain in the verification experiment was 0.765 g.These results provide scientific basis and technical support for the industrial seedling cultivation and development utiliza-tion of edible Lilium resources.
Keywords：Edible lilyMedicinal lilyTissue cultureFactory seedling breedingResponse surface methodology
Publication Date：2025-09-05
Online Publishing Date：2025-09-29(First online date of this platform, not the publication date of the document)
Pages：12( 169-179,186 )