Hybrid purity identification of Brassica campestris ssp. chiensis variety Chunfu by SSR marker
SUN Zhongwei
LÜ Chenchen
XU Youlian
LI Manman
GUO Tao
GAO Qihui
Abstract：Currently,the assessment of seed purity in Brassica campestris primarily relies on field identification methods.These methods are not only time-consuming and labor-intensive,but also susceptible to environmental factors.To ensure the quality of seeds entering the market and to expedite the market penetration of Chunfu seeds,this study developed an efficient identification method for Chunfu seeds by molecular marker technology.To develop an efficient method for iden-tification the seed purity of Brassica campestris(syn.Brassica rapa)ssp.chiensis variety Chunfu,three SSR primers were selected from 96 SSR primer pairs that exhibited polymorphism between the parental lines of Chunfu.Among these,Ra-pa57 demonstrated superior performance and deemed suitable for purity identification.The Rapa57 was selected to identi-fy the seeds purity of Chunfu.Molecular detection matched field phenotypic identification with 1.9％deviation,the con-sistency was 98.1％.These results indicated that SSR marker can be applied to identify the purity of Brassica campestris.This method approach enhances identification efficiency,exhibits excellent stability and accuracy,also provides a robust scientific basis for seed production and quality control.
Keywords：Brassica campestris ssp.ChunfuHybridPurity identification
Publication Date：2025-08-05
Online Publishing Date：2025-09-01(First online date of this platform, not the publication date of the document)
Pages：5( 161-165 )