Resistance evaluation of six tomato rootstock varieties to Fusarium wilt and salt stress

SUN Qingzhen; HE Manli; LIU Hengxin; YANG Chenyu; SHEN Shunshan; PIAO Fengzhi; DU Nanshan

doi:10.16861/j.cnki.zggc.2025.0267

Abstract：In order to address the frequent occurrence of Fusarium wilt and the decline in yield and quality caused by soil salinization in tomato cultivation,this study conducted screening of tomato rootstock resistant to wilt disease and salt tolerance.Using the wild-type tomato Ailsa Craig as the control,the resistance to Fusarium wilt and salt stress in seedling stage of commercial tomato Fendu Golden Crown King and 6 rootstock cultivars were identified.The methods of artificial inoculation of Fusarium wilt and root irrigation with 250 mmol·L-1 NaCl solution were used to conduct phenotypic analy-sis and physiological index determination through pot experiment,and the disease resistance and salt tolerance of the test materials were comprehensively evaluated.The results showed that after 14 days of inoculation with Fusarium wilt patho-gen,Strong Rootstock 73 exhibited significant resistance to Fusarium wilt disease,with a disease index of 14.17％,which was significantly lower than the control and other rootstock varieties.Compared with AC and commercial cultivars,the disease index of the other six rootstock varieties decreased by 82.17％and 69.63％,respectively.The disease index of the other six rootstock varieties ranged from 33.75％to 79.17％,indicating low resistance.At the same time,the antioxidant enzyme activity and the expression levels of pathogenesis-related protein genes(PR1 and PR5)in Strong Rootstock 73 were significantly higher than those in Qiangzhen 100 and Strong Rootstock 74,which were positively correlated with its disase resistancet.After 14 days of salt treatment,the salt damage index of Strong Rootstock 74 was 18.33％,which was 68.21％and 42.12％lower than that of AC and commercial cultivars,respectively,demonstrating strong salt tolerance.The content of malondialdehyde,relative conductivity,superoxide anion content,and hydrogen peroxide content in Strong Rootstock 74 were significantly lower than those in Strong Rootstock 100 and Strong Rootstock 73,while the ac-tivity of antioxidant enzymes increased significantly,which was positively correlated with its salt tolerance.The tomato rootstocks screened out in this experiment for resistance to Fusarium wilt,Strong Rootstock 73 and salt tolerance tomato rootstock Strong Rootstock 74,provide high-quality materials for tomato stress-resistant cultivation and lay important ma-terial for subsequent study.