Comparison of ploidy identification methods for regenerated plants from pepper anther culture

CHENG Zhifang; LI Li; SHI Yanyan; HAN Yanan; CHANG Xiaoke; YAO Qiuju

doi:10.16861/j.cnki.zggc.2024.0799

Abstract：In order to explore an accurate,efficient,and applicable ploidy identification method for regenerated plants from pepper anther culture,in this study,60 regenerated plant lines from 10 genotypes of pepper anther culture were used as the test materials.The ploidy identification was conducted by adopting the plant fertility detection method and DNA flow cytometry,combined with the chromosome counting method of root tip cell squash.The advantages and disadvantag-es of different methods were compared.The results indicated that the accuracy rate of ploidy identification by DNA flow cytometry was 96.67％,this method was rapid and convenient,but it had high cost and was prone to peak bias.The pollen vitality detection method had an accuracy rate of 100％in determining the odd-even ploidy of regenerated plants' chromo-somes,but it could not distinguish the specific ploidy of regenerated plants.The combination of pollen vitality detection method and DNA flow cytometry detection method could significantly reduce the identification cost,improve the accura-cy rate and efficiency.That is,for the lines that have already flowered,the pollen vitality detection method could be used to determine the odd-even ploidy of chromosomes,which was convenient for early chromosome doubling treatment;for the lines that flowered later,the DNA flow cytometry detection method should be used in the seedling stage.If there were difficult-to-determine situations such as peak bias,the pollen vitality could be combined to determine the ploidy of regen-erated plants.