Differential expression of AMPK in different stages of growth and devel-opment of Pleurotus ostreatus and under abiotic stress

LI Huihui; CHEN Zhaoyang; ZHENG Xiukun; WEN Qing; LIU Qing; QI Yuancheng; WANG Fengqin; HU Yanru; SHEN Jinwen

doi:10.16861/j.cnki.zggc.2025.0011

Abstract：To explore the expression characteristics of the three subunits α,β,and γ of AMPK(AMP-activated protein ki-nase)during different stages of the growth and development of Pleurotus ostreatus and under various abiotic stresses,this study first obtained the sequences of the three subunits of AMPK in P.ostreatus through homology alignment.Then,qP-CR was used to investigate the relative expression levels of the three subunits of AMPK during different stages of growth and development,as well as under nutritional stress,temperature stress,heavy metal stress,oxidative stress,and salt stress.The results showed that the AMPKα subunit was highly expressed during the initial stage of fruiting body develop-ment,the primordium stage,the fruiting body stage of P.ostreatus,as well as under temperature stress,heavy metal stress,and oxidative stress.The AMPKβ subunit was highly expressed during the initial stage of fruiting body development,un-der nutritional stress,heavy metal stress,and salt stress.The expression level of the AMPKγ subunit only increased during the initial stage of fruiting body development and under heat stress within temperature stress.The above results indicate that the three subunits of AMPK respond to different environmental stresses and may have different regulatory functions.Among them,the AMPKα subunit plays a key role in the regulatory function of AMPK.This study lays a foundation for further in-depth exploration of the functions of the three subunits of AMPK during the growth and development of P.os-treatus and under different stresses,and also provides innovative ideas for the study of the stress resistance of P.ostreatus.