Effects of Rumex dentatus enzymes pretreatment on physiological and biochemical characteristics of pepper seedlings under heat stress
LI Jinling
LI Baixue
WANG Yichao
YAO Xiang
MA Yongbin
DU Qingjie
WANG Hu
WANG Xueting
LI Juanqi
WANG Jiqing
LI Meng
XIAO Huaijuan
Abstract：To explore the role of plant enzymes in heat damage,the pepper variety Haoyoumingyue was used as the exper-imental material,and five treatments were set up:Using clean water as the control(CK),and Rumex dentatus enzymes were diluted 50-fold(T50),100-fold(T100),200-fold(T200),and 400-fold(T400),respectively.When the two cotyle-dons of the pepper seedlings were fully expanded,the Rumex dentatus enzyme treatment was carried out.After 21 days,the seedlings were transferred to a high-temperature environment of 45℃ for 3 days.By measuring the physiological indicators such as MDA and H2O2 content,the activity of three antioxidant enzymes(SOD,POD,and CAT),and the expression levels of heat-stress-related genes in the leaves of pepper seedlings after root application,the effects of Rumex dentatus enzymes on the physiological and biochemical characteristics of pepper seedlings under heat stress were clari-fied.The results showed that compared with CK,the T100 treatment could effectively increase the relative water content and the activity of SOD and CAT in pepper seedlings under heat stress,and at the same time,it also reduced the POD activity,the content of MDA,H2O2 and proline,and the production rate of O2-·.The results of fluorescence quantitative PCR indicated that the Rumex dentatus enzyme treatment enhanced the tolerance of pepper seedlings to heat stress by reg-ulating the expression levels of antioxidant-related genes CaPOD,CaSOD,CaCAT and heat-stress-related genes CaHs-fA2,CaHSP16.4,CaHSP70.1,CaWRKY55.In conclusion,the exogenous Rumex dentatus enzyme treatment can alleviate the damage caused by heat stress to pepper seedlings,and the effect of the Rumex dentatus enzyme diluted 100-fold is the best.
Keywords：PepperHeat stressRumex dentatus enzymesPhysiological and biochemical characteristics
Publication Date：2025-07-05
Online Publishing Date：2025-09-12(First online date of this platform, not the publication date of the document)
Pages：9( 157-165 )