The seed purity identification of Chinese cabbage Xinxiangxiaobao 23 by InDel molecular marker
XU Shijing
WANG Xiaoling
XIAO Yan
YUAN Ranghua
YANG Chen
JI Keying
Abstract:To rapidly detect the purity of hybrid seeds from frozen damaged plots in the overwintering seed production base of Xinxiangxiaobao 23,ensure that the quality of seeds entering the market meets standards,protect consumers rights,and improve the efficiency of hybrid seed screening,specific primers were selected from 12 pairs of InDel primers for Chinese cabbage to distinguish Xinxiangxiaobao 23 and its parents,and seed identification was carried out.The results showed that three pairs of primers exhibited co-dominance,among which one pair could effectively distinguish Xinxiangxiaobao 23 from the other three similar varieties.This primer was verified in 108 hybrid progenies,and the purity identification result was 93.5%,which was basically consistent with the field identification result.In conclusion,the InDel marker BrID90105 selected in this study can be used for rapid identification of seed purity in Xinxiangxiaobao 23,promoting the healthy development of the seed industry.
Keywords:Chinese cabbageXinxiangxiaobao 23InDel molecular markerSeed purity identification
Publication Date:2025-07-05
Online Publishing Date:2025-09-12(First online date of this platform, not the publication date of the document)
Pages:5( 49-53 )
China Cucurbits and Vegetables

China Cucurbits and Vegetables

PKU
ISSN:1673-2871
Year, Vol.(Issue):2025,38(7)