Establishing of a wet-cold tolerant identification method for cucumber during seedling stage by wet-cold injury index
DING Yuanyuan
LIU Hanqiang
PAN Yupeng
MENG Huanwen
CHENG Zhihui
Abstract：To establish a wet-cold tolerant identification method during seedling stage for cucumber by morphology,the seedlings in two developed true leaves of 8 test cultivars and 5 validation cultivars with different wet-cold tolerance were treated under 9 ℃/5 ℃(day/night)+relative humidity 95％growth chambers(taking 25 ℃/18 ℃+relative humidity 85％as the control).After 7 days of treatment,the degree of wet-cold injury was counted for each plant,the wet-cold injury in-dexes were calculated and the growth parameter were measured.The results showed that the different cultivars presented different level of leaf yellowing,water losing wilting or death under wet-cold stress and the wet-cold injury indexes could indicate the wet-cold tolerance of the cultivar accurately.The results of validation test were consistent with those of meth-od establishment test.The clustering of wet-cold tolerance of various varieties based on the relative values of plant height,leaf length and leaf width etc.under wet-cold stress and control condition were consistent with the classification of wet-cold injury index.Therefore,it is thought that the wet-cold injury index at seedling stage can be used to identify the wet-cold tolerance of cucumber cultivars.The wet-cold injury index greater than 0 but less than or equal to 0.250 indi-cates strong tolerant type,greater than 0.250 but less than or equal to 0.550 indicates moderate tolerant type,greater than 0.550 but less than or equal to 0.750 indicates weak tolerant type,and greater than 0.750 but less than or equal to 1.000 in-dicates sensitive type.
Keywords：CucumberCultivar germplasmWet-cold toleranceWet-cold injury indexIdentification method at seedling stage
Publication Date：2025-06-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 84-90 )