Gene mapping and physiological study of resistance to Botrytis cinerea in gooseberry tomato

JIANG Jianing; XUE Dongqi; LOU Xueyuan; JIANG Liwei; GUO Xin

doi:10.16861/j.cnki.zggc.2025.0164

Abstract：Gray mold,caused by Botrytis cinerea,poses serious threats to both tomato production and post-harvest preser-vation.This study utilized gooseberry tomato(LA2076),cultivated tomato(LA 1246),and their F2 populations as experi-mental materials to analyze the phenotypic and physiological differences between these two types of tomato varieties.By integrating the initial localization interval obtained through segregation population grouping analysis,22 InDel markers were developed for fine mapping of tomato resistance genes against gray mold.The results indicated that at the same time point after in vitro leaf inoculation with B.cinerea,the lesion area in LA2076 was larger than that observed in LA1246.The MDA content exhibited an initial increase followed by stabilization,with LA2076 displaying a faster rate of increase than LA1246.The activities of POD(peroxidase),CAT(catalase),and SOD(superoxide dismutase)all exhibited initial increase follwed by decrease,with LA2076 reaching higher peak antioxidant enzyme activities at earlier time points than LA1246.Genetic mapping revealed that the gene conferring resistance to gray mold was localized to a 4.29 Mb interval flanked by InDel-1.2 and InDel-4.1 on chromosome 9,which contained 38 genes.This research provides a theoretical foundation for guiding genetic breeding efforts aimed at enhancing tomato resistance to gray mold as well as for further in-vestigations into the genetic mapping and molecular mechanisms underlying this resistance.