Transcriptome sequencing and bioinformatics analysis of melon seed-lings under waterlogging stress
WANG Yanan
JIANG Yanfang
LIU Zefa
DANG Jiancheng
ZHANG Honglei
ZHANG Zixia
Abstract：Waterlogging is one of the most widespread and frequent natural disasters worldwide.As a cucurbit crop,mel-on exhibits relatively poor waterlogging tolerance.When melon cultivation areas experience prolonged rainfall or flood-ing,severe yield losses or even complete crop failure often occur.In this study,a waterlogging tolerant melon high genera-tion M13-06-3,was used as experimental material.Transcriptome sequecing and quantitative real-time PCR(qRT-PCR)were employed to analyze differentially expressed genes(DEGs)in root tissues.The results revealed that 9037 DEGs were screened in M13-06-3 under waterlogging stress,including 4529 upregulated and 4508 downregulated genes.qRT-PCR validation confirmed that the expression patterns of five genes,MELO3C003917,MELO3C020588,ME-LO3C008314,MELO3C025360,and MELO3C021658,were largely consistent wiht the transcriptome data,suggesting their potential role in melon waterlogging tolerance.These findings provide a foundation for further exploration of water-logging resistant genes in melon.
Keywords：MelonWaterlogging stressReal-time fluorescence quantitative PCRRNA high-throughput sequencingDifferentially expressed gene
Publication Date：2025-06-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：12( 14-25 )