ARTP mutagenesis technology was used to screen the Auricularia heimuer strains with strong ability of straw degradation
HAN Chuang
WANG Fengli
YUE Xin
ZHAO Yanshu
DAI Xiaodong
MA Qingfang
Abstract：In order to obtain an efficient straw degradation strain of Auricularia heimuer,ARTP mutagenesis breeding technology were used to obtain 134 mutant strains from the protoplasts of Auricularia heimuer Heiwei 15 strain as muta-genic materials.A total of 84 mutant strains were screened by antagonism test,and then three positive mutant strains(26,119 and 134)were screened out with better cellulose degrading ability than the original strain by Congo red staining and extracellular degradation enzyme activity assay.The results of ISSR(inter-simple sequence repeat)molecular marker test showed that these three strains were genetically different from their mother.The results of this study had scientific guiding significance for the selection of new varieties of Auricularia heimuer with efficient straw degradation and the innovation of cultivation substrates.
Keywords：Auricularia heimuerARTP mutagenesisExtracellular enzymesInter-simple sequence repeat marker
Publication Date：2025-04-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 107-112 )