Relationship of Lentinus edodes quality between chemical composition and enzyme activity of fungus sticks based on the sweet cherry sawdust

WANG Zhaofeng; ZHANG Rui; DU Shiyong; QIANG Ruibin; MA Ruilin

doi:10.16861/j.cnki.zggc.2024.0387

Abstract：This paper studied the relationship between the chemical composition and enzyme activity changes of fungus sticks and the quality of Lentinus edodes,using sweet cherry sawdust as the cultivation substrate.In this experiment,the biotransformation rate of fungus sticks,the changes of chemical composition and enzyme activity of fungus sticks,and the quality-related indexes such as components,total phenols content and DPPH radical scavenging rate of Lentinus edodes in different stubbles was determined.The results showed that the ratio of nitrogen,phosphorus and potassium increased significantly by 128.6％,533.3％and 137.5％,respectively,after color conversion.However,these ratios decreased in the later stage of fruiting.With the color conversion and the number of fruiting stubble added,the proportion of lignin and cellulose in the fungus sticks reduced.In the four-stubble sticks,the proportion of lignin and cellulose was significantly decreased by 67.2％and 55.2％,respectively,while the proportion of hemicellulose increased significantly.The activity of protease,carboxymethyl cellulose and laccase in the fungus sticks decreased with the increase of the number of fruiting stubbles.Compared with the first stubble,the activities of protease decreased by 20.6％,43.0％,and 63.4％in the last three stubbles,the activity of laccase decreased by 22.8％,40.6％and 57.8％,and the activity of carboxy-methyl cellulose decreased by 2.4％,17.2％and 26.9％.The content of potassium and lignin,and the activity of protease,carboxymethylcellulase and laccase in the fungus sticks were positively correlated with the quality indicators of Lentinus edodes,while the hemicellulose in the fungus sticks was negatively correlated with the quality indicators of Lentinus edodes.The bioconversion rate of the fungus sticks was 81.1％.Considering the economic cost and time cost,it is recom-mended to harvest the first three stubbles of Lentinus edodes in cultivation.