Application of KASP-SNP technology in fingerprint construction and seed purity detection of pepper seeds of Lamei No.11
HU Zongwei
ZONG Chen
DAI Lei
WEI Zhouying
DAI Zuyun
YANG Zhongzhou
Abstract：To provide an efficient and reliable method for the germplasm resource identification and purity detection of hybrid seeds of pepper,this study conducted genotyping of the variety Lamei No.11 and its male and female materials using kompetitive allele specific PCR(KASP)technology,which is based on 65 pairs of core single nucleotide polymor-phism(SNP)markers developed from the genomes of pepper.The genetic information of Lamei No.11 and its male and female materials were converted into two-dimensional codes by barcode online generating software,and the construction of fingerprints was completed.CaSNP10 and CaSNP11 were used to detect the purity of Lamei No.11 as 98.4％.The purity of pepper variety Lamei No.11 was also detected using simple sequence repeat(SSR)molecular marker and field identification.This study provided a theoretical basis for the protection of pepper variety rights and the identification of pepper variety purity under high-throughput genotyping.
Keywords：PepperDNA fingerprintCultivar purityKASPSNPSSR
Publication Date：2025-04-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 45-52 )