Optimization of substrate formulation of Naematelia aurantialba substi-tute cultivation
LI Bin
TIAN Fang
JIN Li
LIU Qingchao
Abstract：The J-1 and J-2 Naematelia auriculus strains,which have a large cultivation area in Taikang county,Henan province,were used as research objects to explore the effects of 7 different culture media on the mycelia growth of J-2 Naematelia auriculus mother strain,as well as the mycelia growth,earing rate and biological efficiency of J-1 and J-2 strains on 9 different cultivation substrate formulae of cotton seed shell and wood chips.The results showed that the opti-mal mother culture media medium for J-2 strain was PDA medium with 3％wheat grain juice,which achieved the fastest growth rate of 5.45 mm·d-1,the best mycelial growth,and the highest mycelial growth index of 11 665.37.The difference was significant compared with the other six media,and the addition of corn flour and peptone was not conducive to the mycelia growth of J-2 strain.In the experimental design,the optimal cultivation substrate formula was found to be 30％cotton seed shell,60％wood chips,10％sucrose,7.8％corn flour,1％gypsum,0.2％potassium dihydrogen phosphate,and 0.3％magnesium sulfate.With this formula,both J-1 and J-2 strains achieved a 100％earing rate,with the biological efficiency of 69.31％and 72.24％,respectively,providing a scientific basis for the cultivation of Naematelia auriculus.
Keywords：TremellaMother culture mediumHyphal growthCultivation mediumBiological efficiency
Publication Date：2025-03-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 114-118 )