Genome-wide identification and expression analysis of the CmSULTR gene family in melon

Mairebaike·Muhamaitihan; Saierlan·Jianaerbieke; WEI Zhiqiang; WANG Chaonan

doi:10.16861/j.cnki.zggc.2024.0473

Abstract：Sulfate transporter protein(SULTR),as a carrier protein required for active sulfate transport,is a key protein responsible for sulfate absorption and transport in plants.Based on the genomic and transcriptome sequencing data of cantaloupe,the members of the cantaloupe CmSULTR gene family were identified by bioinformatics methods,and their physicochemical properties,chromosome position,subcellular localization,conserved motifs,cis acting elements,gene structure,trans-membrane structure,and expression patterns were analyzed.qRT-PCR validation and analysis were performed in sulfur sensitive and sulfur tolerant materials.A total of 11 SURTR family genes were identified in canta-loupe,and all 10 members of the CmSURTR gene family have complete conserved motifs,indicating high conservation.There are 8-12 trans-membrane structures in the gene structure,and cis acting elements include many hormone responsive elements and stress responsive elements.After sulfur stress treatment,the relative expression level of CmSULTR8 and CmSULTR11 in sulfur sensitive material M446 were significantly higher than those in sulfur tolerant material M531,suggesting that CmSULTR8 and CmSULTR11 may participate in the sulfur tolerance process of melon through negative regulation.The expression patterns of CmSULTR gene family members vary in different tissues,and they synergistically regulate the absorption and transport of sulfate in melon.Among them,CmSULTR1,CmSULTR6,CmSULTR8,and CmSULTR11 may be key genes for sulfate absorption and transport.The above results provide reference for further in-depth research on the SULTR gene family in the growth and development of melon and the mechanism of sulfate transport.