Identification and phylogenetic analysis of cultivable yellow morel mush-rooms
ZHANG Wenchang
SUN Wenhua
WANG Haiyan
YIN Qi
KONG Weili
HE Peixin
LIU Wei
Abstract：The ascocarps of yellow morel samples from a cultivation and domestication base were collected,and mycelial isolates were obtained through tissue separation.Conventional morphological characters combined with the analysis of concatenated sequences of internal transcribed spacers(ITS),elongation factor 1-alpha(EF1-α),RNA polymerase Ⅱsubunit 1/2(RPB1/RPB2)were used to classify and identify three cultivated yellow morel varieties.The macro-and micro-morphological characteristics of the ascocarps supported their identities of yellow morels.Phylogenetic analysis based on four gene fragments showed that the sequences of the three domesticated morel varieties clustered together with 10 morel reference sequences in the Esculenta clade,and with the Mes-21 in a monophyletic group(support rate 100％),indicating that they belong to the Mes-21 phylogenetic species..This is the first confirmed artificial cultivated species of Esculenta clade,which will effectively promote the development of yellow morel artificial cultivation technology and its large-scale cultivation applications in China.
Keywords：Morel mushroomsEsculenta cladeDomesticationPhylogenetic speciesMes-21
Publication Date：2025-02-04
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 59-65 )