Effects of six detoxification agents on the growth of mycelium and relative expression level of LeV-HKB virus in 9608 shiitake mushroom

BAO Dandan; LIU Yanan; DUAN Nini; ZHANG Zhifeng; ZHANG Yingjun; LU Yunfeng

doi:10.16861/j.cnki.zggc.2024.0497

Abstract：To address the issue of varietal degeneration in the cultivation of the 9608 strain of shiitake mushrooms,a study was conducted using PDA medium as a control to investigate the effects of six different detoxifying agents on the growth rate of mycelium,as well as the activity of laccase and carboxymethyl cellulase,and the content of the LeV-HKB virus. The results indicated that compared to the control,the growth rate of mycelium treated with viruszole,1.5 tablets of ber-berine,1 tablet of berberine,honeysuckle,and shiitake polysaccharides increased by 9.33％,1.80％,4.76％,2.44％,and 0.39％,respectively. In contrast,treatments with kanamycin and ampicillin resulted in decreases in mycelial growth rate of 5.26％ and 14.62％,respectively. Furthermore,the enzymatic activity of laccase in the mycelium increased following detoxification treatment compared to the control. The activity of carboxymethyl cellulase after treatment with viruszole,1.5 tablets of berberine,1 tablet of berberine,honeysuckle,and shiitake polysaccharides increased by 39.19％,31.08％,11.49％,10.81％,and 2.70％,respectively,while treatments with kanamycin and ampicillin resulted in decreases of 22.97％ and 0.68％,respectively. Among all treatments,the greatest enhancement in both mycelial growth rate and car-boxymethyl cellulase and laccase activity were observed with viruszole treatment. RT-PCR and Qrt-PCR analyses of my-celium grown on different media revealed that viruszole treatment essentially eliminated the LeV-HKB virus. In conclu-sion,viruszole treatment significantly enhances the growth rate of shiitake mycelium,increases the activity of carboxy-methyl cellulase and laccase,and effectively eradicates the LeV-HKB virus,providing a scientific basis for the high-quali-ty production of the 9608 strain of shiitake mushrooms.