Promoter cloning and expression analysis of sucrose transporter gene StSUT2 in potato
GONG Huiling
YANG Yaqian
LIANG Jinyong
TIAN Ze
FENG Zaiping
BAO Jingting
Abstract：Plant sucrose transporter(SUT)mainly mediates the transport of sucrose extracellular vesicles and plays an im-portant role in plant growth and development. To investigate the expression characteristics of sucrose transporter gene StSUT2,cloned the upstream 1700 bp promoter sequence of the StSUT2 gene using TOPO and performed bioinformatics analysis on it;and the expression pattern of StSUT2 gene in different organs and tissues after different light time was ana-lyzed by qRT-PCR technology. The results showed that there are several photo-responsive elements,and other response el-ements such as abscisic acid,salicylic acid,auxin,anaerobic induction,defense and stress response in StSUT2 promoter region. StSUT2 has the highest expression level in flowers and old leaves after 4 h lighting,and in flowers after 16 h light-ing,while StSUT2 has a certain expression level in young leave,mature leave,stem,root and stolon. Except for the old leave,StSUT2 expression level in various organs and tissues is not significantly affected by the light time. Furthermore,StSUT2 expression level was significantly higher in stamen and pistil than in whole flower,which suggests that StSUT2 plays an important role in the development of flower organs,especially in stamen tissue. These results provide a reference for intensive investigation of the biological function of StSUT2.
Keywords：PotatoStSUT2 promoterCloningExpression pattern
Publication Date：2025-01-04
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 25-30 )