Identification of NPR gene family and its expression under salt stress in watermelon

JIA Yunhe

doi:10.16861/j.cnki.zggc.2024.0618

Abstract：Using Arabidopsis NPR gene family members as query sequences,watermelon NPR gene family members were identified in watermelon genome database,and their bioinformatics and expression under salt stress were analyzed. A total of four members were identified and named ClNPR1-ClNPR4. Their physicochemical properties,conserved functional do-mains,important amino acid residues and motif analysis had high consistency with those reported in other species. Cis-act-ing element analysis showed that NPR gene was associated with abiotic stress in watermelon. Salt-tolerant and salt-sensi-tive material were treated with 300 mmol·L-1 NaCl,and the expression patterns of 4 NPR genes were analyzed in leaves un-der salt stress for 0,8 and 24 h. The results showed that the expression of ClNPR1 was significantly up-regulated in both salt-sensitive and salt-tolerant materials,but the expression of ClNPR1 was significantly down-regulated in salt-sensitive materials for 24 h under salt stress. The expression level of ClNPR1 was significantly higher in salt-tolerant materials than in salt-sensitive materials. After 24 h of salt stress,ClNPR2 and ClNPR3 were significantly up-regulated in both salt-sensi-tive and salt-tolerant materials,and their expression level in salt-tolerant materials were significantly higher than those in salt-sensitive materials. It is speculated that ClNPRs play an important role in watermelon response to salt stress.