Scanning electron microscopic observation and viability test of pollen grains of Zanthoxylum planispinum var.dintanensis
LUO Hui
ZENG Qinmeng
LU Zhongke
HOU Na
LOU Li
WANG Gang
Abstract：The morphological observation and,pollen vitality analysis of the Zanthoxylum planispinum var.dintanensis flower were conducted using an Eclipse E200 biological microscope and an EM-30 Plus scanning electron microscope.This study aimed to provide a scientific basis for the hybrid breeding of Zanthoxylum bungeanum.The results showed that there was a phenomenon of monoecious growth in the Zanthoxylum planispinum var.dintanensis.Upon maturation,the anthers turned bright yellow,and the pollen grains were elongated spheres with fine mesh patterns on the surface.Signifi-cant variations were observed among different individual plants,specifically,the pollen grain diameter of No.1 was the largest(522.32 pm),while that of No.3 was the smallest(279.50 pm).The average length of the polar axis,equatorial axis,pollen size,and mesh diameter of the pollen were 26.76,16.39,442.56,and 0.81 pm,respectively.Pollen vitality tests revealed that No.1 and No.2 exhibited high vitality,with Alexander staining rate of 44.37％and 48.57％,respective-ly,although their associated plant growth was relatively weak.Conversely,the pollen vitality of Individual No.5 was low,with an Alexander staining rate of only 6.67％,while the overall growth of the plant appeared normal with no signs of weakening.These research findings provide a biological basis for understanding the reproductive strategy of the Zanthoxy-lum planispinum var.dintanensis.
Keywords：Zanthoxylum planispinum var.dintanensisMorphological structurePollen sizePollen vitality
Publication Date：2024-10-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 118-123 )