Construction and application of paper test strip for rapid detection of pesticide residues
SUN Zhaowei
ZHAO Xiaozhong
HU Bo
ZHAO Yihang
XU Xue
DU Miao
WENG Haibo
Abstract：Simple and rapid screening of organophosphorus and carbamate pesticide residues is of great significance for food safety.In this study,the authors designed and developed a one-step rapid test strip using enzyme inhibition method,with the diazo red RC salt as indicator and indoxyl acetate as substrate of acetylcholinesterase(AChE).The bottom of the test strip is a plastic rubber plate,which is successively pasted with sample pad,enzyme pad,block pad,substrate pad,adsorption pad and nitrocellulose(NC)film that fixed with diazo red RC salt.Qualitative or quantitative analysis of pesti-cide residues can be achieved by detecting changes in the color of the detection area,and the operation method is simple and fast.The detection limits of different pesticides are as follows:0.6 mg·L-1 for malathion,0.4 mg·L-1 for omethoate,1.3 mg·L-1 for acephate,0.1 mg·L-1 for dichlorvos,0.1 mg·L-1 for carbofuran,0.07 mg·L-1 for aldicarb.It shows good reproducibility and high sensitivity in actual fruit and vegetable samples.
Keywords：AcetylcholinesterasePesticide residueRapid detectionTest strip
Publication Date：2024-06-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 111-118 )