Resistance physiological study and evaluation system establishment on Fusarium wilt of bitter gourd

LING Yi; YANG Yan; NIU Yu; YU Renbo; LIU Zhaohua; LIU Pingwu; HAN Xu

doi:10.16861/j.cnki.zggc.2024.0056

Abstract：Through the study of bitter gourd Fusarium wilt resistance physiology,the authors have constructed an effi-cient and precise identification and evaluation system for bitter gourd Fusarium wilt resistance,aiming to provide a the-oretical basis for the screening and evaluation of Fusarium-resistant germplasm resources and the selection and breeding of disease-resistant varieties.In this study,Reke No.2 bitter gourd was used as the test material,and selected the root irrigation,radicle root and injure root methods to inoculate different concentrations of Fusarium wilt fungus liquid at the time of two-leaf and one heart of bitter gourd,and investigated the effects of different inoculation methods and inocula-tion concentrations on the Fusarium wilt by analyzing the differences in disease index and physiological responses at different inoculation times.The results showed that the disease index was 37.1 using the root irrigation inoculated fungus with 1× 106·mL-1 at 25 d,which was significantly lower than that of 1×107·mL-1,and significantly higher than that of 1×104·mL-1.PAL activity and chlorophyll content were significantly lower than the radicle method,and no significant difference existed between PPO activity and soluble protein content.When bitter gourd seedlings of two-leaf and one heart were inoculated with 1×106·mL-1 fungus using the root irrigation method,the disease incidence was identified at 25 d,which can serve as an accurate and efficient identification system of bitter gourd Fusarium wilt resistance.