Analysis of differential metabolites during fruiting body development of Pleurotus ostreatus based on untargeted metabolomics
LIU Qin
HUANG Bao
HU Sujuan
NIU Senyuan
WU Jie
ZHOU Aoshuo
KONG Weili
Abstract：In order to understand the metabolite basis of fruiting body formation during the growth and development of Pleurotus ostreatus,ultra performance liquid chromatography-tandem mass spectrometry interfaced with electro-spray ionization(UPLC-ESI-MS/MS)combined with multivariate statistical methods was used to analyze the differences of in-tracellular metabolites in mycelia of P.ostreatus at the spawning completion stage,primordium stage and fruiting body differentiation stage.The results showed that principal component(PCA)model revealed significant differences(p＜0.05)among metabolites in mycelia of P.ostreatus at the three periods.Through orthogonal partial least squares discrimi-nant analysis(OPLS-DA),139,147 and 67 differential metabolites were screened and identified in MM vs MP,MM vs MF and MP vs MF under the conditions of VIP(varible importance in the projection)＞1 and fold change≥2 or ≤ 0.5.Amino acids and derivatives,lipids,alkaloids and organic acids were substances with the maximum fold change values,which indicated that these differential metabolites played important roles in the fruiting body development.KEGG analy-sis showed that phenylalanine metabolism,tryptophan metabolism,purine metabolism and other metabolic pathways were active.During the growth and development of P.ostreatus,the contents of lipids,organic acids,nucleotides and their de-rivatives,and amino acids and their derivatives were significantly correlated.The results of this study provided theoretical basis for the mechanism of fruiting body development and standardized cultivation of P.ostreatus.
Keywords：Pleurotus ostreatusMetabonomicsMyceliaDifferential metabolitesFruiting body development
Publication Date：2024-01-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：11( 45-55 )