Inheritance and sequence variation analysis of a wax-less mutant YW71 in Chinese cabbage(Brassica rapa L.ssp.pekinensis)
YANG Shuangjuan
TANG Hao
ZHAO Yanyan
WEI Xiaochun
WANG Zhiyong
SU Henan
ZHANG Wenjing
LI Lin
WANG Zuojing
YUAN Yuxiang
ZHANG Xiaowei
Abstract：In this study,we identified a wax-less Chinese cabbage(Brassica rapa)mutant YW71,the inheritance and the controlling gene for the glossy trait were studied extensively.Genetic analysis indicated that the glossy trait in YW71 was controlled by a single recessive locus.Allelic complementary experiment showed that the glossy trait in YW71 was caused by mutation of the gene BrWAX2.Sequence analysis revealed that a 39 bp deletion was identified at the end of the third exon of BrWAX2,resulting in alternative splicing at the transcription level and finally leading to a premature stop codon at the translation level.Expression analysis showed that BrWAX2 was significantly down-regulated in stems and leaves of glossy YW71.Furthermore,a co-dominant marker BrWAX2-InDel was developed and validated.Overall,these results enrich the genetic resources of glossy mutants and provide applicable markers for marker-assisted selection(MAS)-based breeding of Chinese cabbage,which has pivotal significance in theory and practice.
Keywords：Chinese cabbageGlossy mutantAllelic complementary experimentSequence variation analysis
Publication Date：2024-01-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 32-38 )