Evaluation of resistance of Chinese chive gray mold(Botrytis cinerea)against pyrimethanil and fludioxonil and the suitability of resistant strains
ZHANG Yanli
CHEN Juan
ZHANG Yixiao
CHEN Yanling
Abstract：In order to clarify the sensitivity level of the pathogen of Chinese chiv gray mold to pyrimethanil and fludioxo-nil in different regions of Gansu province,fifty-four strains were collected and isolated from the disease plants in differ-ent regions.In order to divide the sensitive and resistant strains,the minimum inhibitory concentrations of pyrimethanil and fludioxonil were determined by the MIC method(minimum inhibitory concentration method).The susceptibility of resistant strains to pyrimethanil and fluxonil was determined by mycelium growth rate method,and their resistance levels to the two fungicides were also tested.The genetic stability and fitness of resistant strains were further analyzed.The mini-mum inhibitory concentrations of pyrimethanil and fludioxonil against fifty-four strains of Botrytis cinerea were 4.8,0.15 μg·L-1,respectively.The resistance frequency was 35.19％and 9.26％respectively.The resistance of the pathogen of B.cinerea to pyrimethanil was medium and high,low resistance to fludioxonil.The low resistance strains against pyrimethanil can not be inherited stably.However,there was a trend towards greater sensitivity to pyrimethanil after subculture.The suitability of pyrimetrium-resistant strains was significantly lower than that of pyrimetrium sensitive strains.The pyrimetrium-resistant strains were not superior in competition with sensitive strains.The suitability of the fluroxonitrile resistant strains was similar to that of the fluroxonitrile sensitive strains.
Keywords：Chinese chivesGray moldPyrimethanilFludioxonilResistance evaluationFitness
Publication Date：2023-12-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 107-112 )