Molecular identification of the cytoplasmic male sterile types in flower-ing Chinese cabbage
REN Zhiyong
NIE Qijun
DONG Binfeng
HU Zhiwei
ZHANG Junhong
WANG Kun
LI Jinquan
Abstract：Cytoplasmic male sterility(CMS)has been widely applied in flowering Chinese cabbage breeding.To improve the accuracy and efficiency of CMS type identification and investigate the CMS application in flowering Chinese cab-bage,the specific multiplex PCR CMS marker Brcms_M for simultaneously detecting Polima,Ogura and Hau CMS was developed.Brcms_M could overcome the false positive problem in Polima CMS identification when using the reported Polima markers in Hau CMS materials,and also could distinguish three CMS and normal cytoplasmic type.Brcms M was furtherly used to identify the CMS type of the twenty-eight flowering Chinese cabbage varieties.The result of CMS type identification indicated that Polima CMS was the mainly applied in purple flowering Chinese cabbage breeding,and CMS type of white flowering Chinese cabbage and flowering Chinese cabbage majorly belong to the Ogura CMS.Inter-estingly,two partial sterility varieties were also identified as Ogura CMS type,Ogura CMS restorer gene Rfo was not found in these two varieties.
Keywords：Flowering Chinese cabbageCMSMultiplex PCRMolecular marker
Publication Date：2023-12-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：8( 39-46 )