Hybrid purity identification of Chinese cabbage Bianzao No.9 by InDel marker
FENG Jianqi
WANG Peiyun
CAI Yaping
DING Cong
WANG Huiyun
SHENG Yuanyuan
Abstract：To identify seed purity of Chinese cabbage(Brassica rapa)Bianzao No.9 accurately and efficiently,32 pairs of published InDel core primers based on the whole genome of Chinese cabbage were used.The InDel primer BrID90029 was selected to identify the seeds purity of Bianzao No.9.The bands showed diversity between parents and were comple-mentary with high clarity.Based on the primer,288 Chinese cabbage F,hybrids Bianzao No.9 were identified for purity.Molecular detection matched field phenotypic identification with 99.29％consistency.Therefore,the InDel primer BrID90029 can be applied to accurately and efficiently identify the purity of Chinese cabbage hybrid Bianzao No.9.This study provides a technical basis for large-scale detection of the purity of Bianzao No.9,and has a significant application value.
Keywords：Chinese cabbageBianzao No.9F1 hybridPurity identification
Publication Date：2023-12-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 33-38 )