Screening,identification and biocontrol effect of antagonistic bacteria against melon Fusarium wilt
HAO Fangmin
DONG Wenjie
ZANG Quanyu
MA Erlei
DING Weihong
WANG Yuhong
Abstract：To obtain effective biocontrol fungi against melon pathogens,dilute coated plates and molecular identification methods were used,strain Be 1-20 was isolated from the melon greenhouse planted for many years,and was identified as Pseudomonas aeruginosa.In order to screen the strains that have antagonistic and growth promoting effects,the experi-ments of plate confrontation,root irrigation and pot inoculation method were carried out.The result showed that Bcl-20 had good inhibitory effect on the pathogen of various fungal diseases of melon,such as Fusarium oxysporum,Monos-porascus cannonballus,Stagonosporopsis cucurbitacearum,F.solani and F.asiaticum,and showed strong antagonistic activity on the plate.Furthermore,P.aeruginosa Bcl-20 could promote the growth of melon seedlings,and significantly increased the stem diameter,fresh weight,length and width of cotyledon and the length and width of the largest true leaf of melon seedlings compared with the control.In addition,pot experiment also showed that it had biocontrol potential to control the melon fusarium wilt and the relative control effect was 52.34％.P.aeruginosa Bcl-20 has the potential of biocontrol and can effectively control muskmelon wilt.
Keywords：MelonPseudomonas aeruginosaBcl-20Fusarium wiltBiocontrol potential
Publication Date：2023-12-05
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 26-32 )