Rapid identification of the purity of watermelon hybrids by SSR markers
ZHANG Peilun
GUO Luqin
HU Qianmei
YANG Luming
MA Changsheng
Abstract：SSR markers are specific DNA fragments which can detect certain differences between individuals or populations at the DNA level.In order to identify quickly and efficiently the purity of hybrids,a perfect SSR molecular marker identification technology system for watermelon hybrids was established.In this study,30 pairs of SSR primers were used to screen the polymorphism of ‘Yuyi 360’,‘Yuyi Heixiaobao’,‘Guoyu 2’ and ‘Tianwang’ 4 which were the representative watermelon hybrid parents,7 of these primers were selected with clear bands and good polymorphism according to the test.The PCR reaction system was amplified and the gel electrophoresis was used for purity analysis.550 F1 samples of four hybrids were used to verify the accuracy of the markers,and the purity was 91.1％,93.2％,92.6％ and 91.4％,respectively.The results were identified by molecular markers and field morphology.The results showed a high degree of agreement and the errors were within the allowable range of ≤2％.Therefore,SSR molecular marker can be used to replace the identification of traditional field morphological characters and identify rapidly the purity of watermelon hybrids.
Keywords：WatermelonSSRMolecular markerPurity identification
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 16-19 )