Comparisons of DNA extraction in Cucurbita moschata Duch.
CHEN Xuejin
GUO Weili
JIANG Lina
LI Xinzheng
ZHOU Junguo
Abstract：The DNA extraction is a basic experimental protocol of gene cloning,PCR reaction,molecular hybridization and genetic polymorphism analysis.Whatman FAT card has been widely applied on DNA conservation and extraction with high efficiency and simplicity.In this study,pumpkin (Cucurbita moschata Duch.) root,stem and leaf were used to extract DNA using boiling method and FTA method,using CTAB method as reference,aiming to explore a simple and efficient DNA extraction method.The results indicated that the DNA quality extracted by using CTAB method was optimal compared to the other two methods and the bands were clear and bright.However,CTAB method was the most complicated.The DNA quality extracted from boiling method,except leaf,was poor and not fit for PCR even though the protocols was relatively simple.The DNA quality extracted using FAT method was good and fit for PCR with clear and bright bands.FTA method is optimal since the protocol is simple and efficient.
Keywords：Cucurbita moschata Duch.DNA extractionCTABBoiling methodFTA card
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 17-19 )