The use of SSR markers for analyzing the genetic relationship among watermelon inbred lines
XU Yanbin
WANG Yanling
WEN Xinhui
HU Jianbin
LI Qiong
SUN Shouru
MA Changsheng
Abstract：The present study aims to identify the relationship among the watermelon inbred lines by using SSR markers.Two markers,SSR and EST-SSR,were used to analyze the genotypes of the 34 inbred lines,which was adopted to calculate the similarity coefficient among the accessions and then cluster the accessions.The resuhs showed that 9 genomic SSRs and 8 EST-SSRs detected 83 alleles totally,with a mean of 4.88.The PIC value ranged from 0.112 to 0.686,averaging 0.425.The polymorphism level of genomic SSR was higher than EST-SSR.All the materials were clustered into 3 groups (A,B and C).of the groups,B and C contained 4 accessions (X36,X48,X84,and X89) with high polymorphism,which were small fruit type accessions collected from northwest region.The 4 accessions could be used to increase the polymorphism of the cultivated watermelon.Group A contained the rest 30 domestic and foreign inbred lines which fruit weight no less than 1.5 kg with an exception of X38 and X80.These accessions had a low polymorphism and the genetic background need to be expanded further.Group A could be subdivided into 3 subgroups,each of which contained accessions with different origins and botanical characters.The results obtained here will offer theoretical basis for parent selection of watermelon cross breeding and new germplasm creation.
Keywords：WatermelonInbred linesSSRGenetic relationship
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 10-13,35 )