Establishment of the cotyledon node regeneration system and vitro-grafting technology in triploid seedless watermelon
MIN Ziyang
RUAN Wanhui
ZHANG Yi
ZHU Feiying
XIAO Jiling
LI Jiguang
LIU Jianxiong
LIANG Zhihuai
Abstract：In this paper,we used seedless watermelon ‘ Chaoting No.3’ cotyledonary node as explants for the establishment of seedless watermelon cotyledon node regeneration and grafting breeding technology system to investigate the key factors affecting cotyledon node regeneration in vitro and the grafting seedling techniques.The result showed that 3％ of sodium hypochlorite solution of 18 min was the best disinfection technique to seedless embryo.2.5 mg· L-1 6-BA+0.1 mg·L-1 NAA had the best adventitious bud induction in seedless watermelon,the differentiation coefficient of adventitious bud was as high as 6.11.0.2 mg· L-1 KT could effectively induce adventitious bud to elongate.Scions with 2.5 cm in length which were grated to pumpkin root stocks with flatted cotyledon had the the highest survival rate (84.4％).In the experiment,we successfully established the system of cotyledon node regeneration and vitro-grafting technology in triploid seedless watermelon.
Keywords：Seedless watermelonTriploidCotyledon nodeRegeneration systemGrafting
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 8-11 )