Detection of seed- transmitted viruses from dry melon seeds using RT-PCR
PENG Bin
WANG Ligang
GU Qinsheng
Abstract：In order to establish a method of detection of the major seed-transmitted virus in dry cucurbit seeds,we modified the total RNA extract methods based on TRIZOL and CTAB,and then the high quality of total RNA was obtained from dry melon seeds rich in polysaccharide. A duplex RT-PCR method was developed for four kinds of seed-transmitted viruses in?fecting cucurbits respectively,including an internal amplification control 18 S rRNA gene of host. The total RNA extracted from single and mixture(about 20 seeds)infected melon seeds by modified TRIZOL method were used as templates of dul?plex RT-PCR detection for the four kinds of viruses respectively. The results indicated that RT-PCR detection system was capable of detecting four different viruses pathogens in samples,and the 18 S rRNA gene as internal control was a useful in?dicator of the quality of plant total RNA extraction effect and the effectiveness of RT-PCR.
Keywords：CucurbitaceaeDry seedsPolysaccharideSeed-transmitted virusRT-PCR assay
Publication Date：2015-11-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 10-15 )