Study on in vitro Culture of Un-pollinated Ovary in Watermelon
LI Ling
MIN Zi-yang
TONG Long
LI Han
CHENG Juan
SUN Xiao-wu
Abstract：Un-pollinated ovaries of‘Zaochunhongyu’,‘Xinong No. 9’and‘Xiaolühuang’used in this in vitro culture experiment. Factors of heat shock treatment of donor plants in dark,genotype,sampling time,2,4-D concentration and combinations of various hormone concentration were tested in this study. Heat shock treatment in dark under 33℃for 4 days was the best treatment for in vitro culture of unfertilized ovary. The un-pollinated ovary one day before flowering had the highest response frequency 17.2%. Among genotyped tested only‘Zaochunhongyu’regenerated plant. The best growth regulator combination for‘Zaochunhongyu’was 2,4-D 4.0 mg·L-1+6-BA 2.0 mg·L-1+NAA 0.5mg·L-1,produced regeneration frequency of 15.0%. The regenerated plants included haploid,diploid and tetraploid based on cytological analysis.
Keywords：WatermelonUn-pollinated ovaryInduction rateIn vitro cultureHaploidInfluencing Factors
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 6-10 )