Transformation System Through Agrobacterium tumefaciens Mediated Unfertilized Ovary Culture in Cucumber
WEI Ai-min
DU Sheng-li
HAN Yi-ke
LIU Nan
Abstract：A new transformation technology through Agrobacterium tumefaciens mediated cucumber unfertilized ovary cul-ture was studied. Herbicide glufosinate-ammonium was used as the selection marker,the optimum herbicide selection pres-sure during embryogenesis was 0.5-1.0 mg·L-1,and optimum selection pressure during plantlet rooting was 2.0 mg·L-1;the optimum carbenicillin concentration for bacteria control was 450 mg · L-1;the optimum infection method was soaking ovary slices into A. tumefaciens solution with OD600 at 0.3 for 10 min,and then co-cultured for 3 days;0.01%surfactant silwet-77 in bacteria solution and co-culture medium was beneficial for transformation. The establishment of this new technology pro-vided a new way for cucumber transformation research.
Keywords：Cucumber(Cucumis sativus L.)Unfertilized ovaryA. tumefaciensTransformation
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 5-9 )