Epidermal growth factor receptor variant Ⅲ promotes calcium ion efflux in glioma cells
JIE Ziyuan
CUI Zhongliang
XU Menghui
LI Guanzhang
YAN Xu
ZHANG Wei
SHI Zhongfang
Abstract：Objective To explore the impact of epidermal growth factor receptor variant Ⅲ(EGFRv Ⅲ)on transmembrane calcium ion(Ca²⁺)flow in glioma cells and its association with temozolomide(TMZ)resistance.Methods Stably transfected U87 and LN229 glioma cell lines expressing EGFRv Ⅲ were established.EGFRv Ⅲ protein and mRNA expression levels were validated using immunofluorescence staining,quantitative real-time PCR(qRT-PCR),and Western blotting.Non-invasive micro-test technique(NMT)was employed to measure transmembrane Ca²⁺flux characteristics.Results Compared with non-transfected controls,EGFRv Ⅲ-transfected U87 and LN229 cells exhibited strong positive immunofluorescence signals for EGFRvⅢ,with significantly elevated mRNA and protein levels confirmed by qRT-PCR and Western blotting(all P<0.001),indicating successful transfection.NMT measurements revealed that non-transfected cells predominantly demonstrated Ca²⁺influx,whereas EGFRv Ⅲ-transfected cells showed a reversal of transmembrane Ca²⁺ flux direction from influx to efflux.Conclusions EGFRv Ⅲ expression significantly alters transmembrane Ca²⁺ flux patterns in glioma cells(from influx to efflux),suggesting that EGFRv Ⅲ may contribute to TMZ resistance in glioma by promoting cellular Ca²⁺efflux.
Keywords：gliomaepidermal growth factor receptor variant Ⅲnon-invasive micro-test techniquecalcium ion
Publication Date：2026-02-20
Online Publishing Date：2026-03-18(First online date of this platform, not the publication date of the document)
Pages：6( 109-114 )