Shikonin combined with temozolomide inhibited the invasion and migration of glioma cells
Li Bo
Liu Binghui
Yang Ming
Hong Weili
Ying Yong
Chen Danlong
Li Yongning
Abstract：Objective To investigate the effects and mechanisms of shikonin combined with temozolomide(TMZ)on the invasion and migration of GL261 glioma cells.Methods GL261 glioma cells in the logarithmic growth phase were used.The effects of shikonin and TMZ on cell viability were detected using the CCK-8 assay to identify the optimal drug concentrations.The experiment was divided into control group,TMZ group,and TMZ+shikonin group(T+S group).Intracellular reactive oxygen species(ROS)were detected by flow cytometry.Cell mobility was detected by scratch assay.The expressions of matrix metalloproteinases(MMP2)and pyruvate kinase M2(PKM2)were detected by Western blotting.Results The optimal drug concentrations were determined to be 400 μmol/L for TMZ and 2.5 μmol/L for shikonin by the CCK-8 assay.Compared with the control and TMZ group,the T+S group showed a significant increase in ROS levels(P＜0.05).After 24 hours of treatment with T+S,the cells showed a dead floating state,the scratch area increased,the cells no longer migrated,and the tumor cells showed a necrosis and apoptosis state.Compared with the control group,the expression levels of MMP2 and PKM2 proteins in the T+S group were significantly decreased(P＜0.05).Conclusions Shikonin combined with TMZ downregulates the expression of MMP2 and PKM2 proteins in glioma cells and inhibits their invasion and migration,which may be related to the upregulation of ROS levels induced by the combination treatment.
Keywords：gliomatemozolomideshikoninreactive oxygen speciesinvasion and migration
Publication Date：2025-02-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 104-108 )