Effects of knockdown of mucosa-associated lymphoid tissue lymphoma translocation protein 1 on the expression of inflammatory factors and neurological function in rats with spinal cord injury
Huang Weiming
Chen Hongquan
Zhang Junxin
Zhang Qingping
Liu Qingyang
Abstract：Objective To investigate the impact of mucosa-associated lymphoid tissue lymphoma translocation protein 1(MALT1)on neuroinflammation and neurological function recovery after spinal cord injury(SCI)in rats.Methods Ninety-six SD(Sprague Dawley)rats were randomly divided into a normal group and a MALT1 knockdown group.The normal group was further divided into a sham group(n=24)and a SCI group(n=24).The MALT1 knockdown group was equally subdivided into 4 groups(12 each):a sham group,a SCI group,a SCI group injected with lentivirus as negative control(SCI+NC group),and a SCI group injected with lentivirus-mediated MALT1 knockdown(SCI+KD group).The rats'motor ability was assessed using the BBB(Basso,Beattie and Bresnahan)score.The severity of SCI was determined by hematoxylin-eosin(HE)staining.Western blot analysis was performed to detect the expression of interleukin-6(IL-6),interleukin-10(IL-10),tumor necrosis factor-alpha(TNF-α),and MALT1 in the spinal cords of rats in each group.Results Compared with the sham surgery group,the SCI group exhibited higher MALT1 expression and neuroinflammatory markers,as well as poorer motor ability(all P<0.05).In the SCI+KD group,MALT1 knockdown improved the BBB scores from 10 to 28 days post-modeling,reduced neurological damage(P<0.05),and decreased the expressions of inflammatory cytokines IL-1β,IL-6,and TNF-α(all P<0.05),but had no effect on IL-10 expression(P>0.05).Conclusions Knockdown of MALT1 improves spinal cord functional recovery by inhibiting the expression of neuroinflammatory factors in SCI.
Keywords：spinal cord injurymucosa associated lymphoid tissue lymphoma translocation protein 1inflammatory factorsneurological function
Publication Date：2024-10-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 603-608 )