Apoptosis of human glioma cells induced by the combination of 5-aminolevulinic acid-mediated sonodynamic therapy and hyperthermotherapy
Wu Hongjie
Ju Donghui
Li Bo
Zhang Weiguang
Duan Xingbang
Chen Huirong
Abstract：Objective To investigate the anti-glioma effects and possible mechanisms of action of sonodynamic therapy(SDT)combined with hyperthermia(HT).Methods The human glioma cell lines SNB19 and U87MG were cultured in vitro and divided into control group,HT group,SDT group and SDT+HT group.Alamar blue assay was performed to assess the inhibiting effect on the proliferation of glioma cells.Apoptotic rate,mitochondrial membrane potential and reactive oxygen species(ROS)were examined by flow cytometer.The effect of SDT+ HT on the expression of apoptosis-related proteins,including cleaved caspase-3,-8,and-9,Bax and Bcl-2,was detected with Western blot.Results Compared with SDT group,the SDT+HT group inhibited glioma cell proliferation,which was positively correlated with 5-aminolevulinic acid(5-ALA)concentration,ultrasound exposure time and HT temperature(all P<0.05),and was accompanied by typical morphological changes of decreased cell viability.Lower dose of HT(42℃ for 15 min)significantly promoted the increase of SDT-mediated intracellular ROS production,the decrease of mitochondrial membrane potential level and the increase of apoptosis(all P<0.05).Compared with the control group,HT group and SDT group,the SDT+HT group up-regulated the protein expression of cleaved caspase-3,-8,-9 and Bax,and down-regulated the expression of Bcl-2 protein(all P<0.05).Conclusions HT may significantly facilitate 5-ALA-SDT to inhibit glioma cell proliferation and induce apoptosis,and then inhibit glioma growth.SDT combined with HT therapy is a promising treatment for glioma.
Keywords：gliomasonodynamic therapyhyperthermotherapyapoptosis
Publication Date：2024-01-20
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 19-23 )