Effects of resveratrol on blood hypercoagulability in glioma animal model after chemotherapy by regulating Wnt pathway and its mechanism
Chang Na
Chen Chao
Zhao Meng
Zhang Rui
Li Bo
Abstract：Objective To investigate the effect and mechanism of Resveratrol(RSV)on blood hypercoagulation in glioma animal model after chemotherapy by regulating Wnt pathway.Methods An animal model of mouse glioma was constructed by subcutaneous injection of U87 cells.The successfully modeled mice were randomly divided into 3 groups:control group(n=5),temozolomide(TMZ)group(n=5)and TMZ+RSV group(n=5).TMZ group and TMZ+RSV group were given TMZ intragastrically with a dose of 50mg/(kg·d).TMZ+RSV group were intervened by RSV.To analyze the effect of RSV on tumor growth and body hypercoagulability state.The level of Wnt pathway in tumor and vascular endothelial tissue was detected by Western blot.Results The tumor volume(144.56±15.24)mm3 and mass(114.11±13.07)mg in TMZ group were significantly lower than control group(P<0.05).Tumor volume(106.45±13.15)mm3 and mass(90.23±10.38)mg in TMZ+RSV group were significantly lower than TMZ group(P<0.05).The apoptosis rate of tumor tissue in control group was(4.16±1.05)%,and that in TMZ group was(15.83±2.54)%,which was significantly higher than that in control group(P<0.05).The apoptosis rate of TMZ+RSV group was(24.03±3.85)%,which was significantly higher than that of TMZ group(P<0.05).Whole blood viscosity,PT,APTT and ET1 in TMZ group were significantly higher than those in control group,while NO was lower than that in control group(P<0.05).Whole blood viscosity,PT,APTT and ET1 in TMZ+RSV group were significantly lower than those in TMZ group,while NO was higher than that in TMZ group(P<0.05).There was no significant difference in Wnt2 and β-catenin in tumor and vascular endothelial tissue between control group and TMZ group(P>0.05),but Wnt2 and β-catenin in tumor and vascular endothelial tissue of TMZ+RSV group were significantly lower than those of control group and TMZ group(P<0.05).Conclusions RSV can promote tumor cell apoptosis and protect vascular endothelial function by inhibiting the Wnt pathway in tumor tissues and vascular endothelial cells,and alleviate the hypercoagulable state.
Keywords：gliomatemozolomideresveratrolwnt pathwayhypercoagulability
Publication Date：2023-12-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 169-173 )