Effect of new permeable dressing in traumatic brain injury rats with seawater immersion
Pan Zhenhong
Zhang Hao
Yang yang
Ye Dan
Xu Yongjun
Huang Yinxing
Wu Zaixin
Wei Liangfeng
Wang Shousen
Abstract：Objectives To investigate the neuroprotective effect of a new permeable dressing in rats of traumatic brain injury(TBI)combined with seawater immersion(SI).Methods A new permeable dressing was prepared by using methylacrylylated gelatin and conductive polypyrrole,and by wrapping normal saline in the hydrogel.36 SD rats were randomly divided into sham-operated group(Group A,n=12),TBI+SI group(group B,n=12)and penetrating dressing group(group C,n=12).The rat models were prepared by cranial percussion apparatus and immersed in artificial seawater for 30min.In the group C,a new penetrating dressing was implanted at the injured site after SI.The neural function of rats was evaluated by modified neurological severity score(mNSS)and open field test 72h post injury.After the behavioral test,the brain water content and Evans blue(EB)content were determined.The morphology of brain tissue was observed by hematoxylin-eosin(HE)staining.The survival of neurons was detected by Nissl staining.The ultrastructural changes of neurons were observed by transmission electron microscope.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of inflammation-related factors(NF-кB,TNF-α,IL-10).Results The new permeable dressing can wrap the saline and permeate the saline at a certain rate,and about 80%of the saline exudates after 45 minutes.Pathological examination showed obvious cerebral edema and severe cerebral hemorrhage in group B.Cerebral hemorrhage and cerebral edema in group C were less than group B.The myelin integrity and survival number of neurons in group C were significantly better than those in group B.Under transmission electron microscopy,mitochondria in group B were reduced,mitochondrial membrane thickened and ridge broken,and chromatin in group C had edge aggregation and mitochondrial damage decreased.Compared with group B,the neural function scores of rats in group C were decreased,and the contents of NF-кB and TNF-α in brain tissue were significantly decreased(P<0.05),IL-10 content was significantly increased(P<0.05).Conclusion The new permeable dressing can effectively promote the recovery of nerve function in rats with traumatic brain injury combined with seawater immersion,and its mechanism may be related to the regulation of inflammatory response.
Keywords：brain injurytraumaticseawater immersionnew permeable dressingrats
Publication Date：2023-12-30
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 164-168 )