HMGB1 expression in lesions of intractable temporal lobe epilepsy

Fu Yu; Wang Benhan; Li Jinglun; Guo Xiaodong; Cao Fuqiang; Liu Wei; Yao Anhui

doi:10.11850/j.issn.1009-122X.2018.05.012

Abstract：Objective To explore the expression features of high-mobility group box 1 (HMGB1) in lesions of intractable temporal lobe epilepsy. Methods The surgical specimens of patients with intractable temporal lobe epilepsy were collected, including non-status epileptic group (NSE, n =11) and status epilepticus group (SE, n =13). Six specimens from thalamic tumor resected via a transcortical approach as control group. The expressions of HMGB1 in the brain tissue and neurons from the lesions were detected. The transfer of HMGB1 from cell nucleus into cytoplasm was counted. Results Expression of HMGB1 in the lesion tissue was 78.1±25.90 in control group, 98.7 ±18.17 in NSE group and 134.1 ±16.56 in SE group. Compared with control group, NSE group showed no significant difference (P =0.051) and SE group increased more significantly (P ＜ 0.05). The ratio of HMGB1-positive cytoplasm was 22.3±11.85％ in control group, 34.5±8.59％ in NSE group and 41.3±4.60％ in SE group. The number of HMGB1-positive neurons was 13.9±2.75 in control group, 25.0±3.54 in NSE group and 48.9±9.85 in SE group. The ratio of HMGB1-positive neuronal cytoplasm was 11.9±4.69％ in control group, 36.4±6.62％ in NSE group and 51.1±7.47％ in SE group. Compared with control group, the ratio of HMGB1-positive cytoplasm, the number of HMGB1-positive neurons and the ratio of HMGB1-positive neuronal cytoplasm in the lesion tissues increased significantly in NSE group (all P ＜ 0.01), and increased further in SE group (all P ＜ 0.01). Conclusion Increase of HMGB1 expression and the ratio of HMGB1 transfer from the nucleus into cytoplasm in the epileptic lesion may closely relate to the pathophysiological process of epilepsy.