Expression and methylation levels of SOX9 gene in human glioma
Wei Chuanjiang
Zhao Tianshu
Fu Changyu
Dong Baijing
Wang ce
Duan Xingbang
Liu Xiaoqian
Abstract：Objective To explore the expression level and methylation status of SOX9 gene in human glioma,and find out the relationship between SOX9 and pathological grade of glioma.Methods Totally 113 glioma tissues and 34 normal brain tissues were collected.SOX9 expression was detected by RT-PCR method and methylation status was measured by PCR-MSP.The survival curve was analyzed by Kplan-Meier methods.Results The relative expression level of SOX9 mRNA was 0.203±0.097 and 0.496±0.213 in normal brain tissues and glioma tissue respectively,and significant difference was found between them (P ＜ 0.01).Survival curve showed that the survival time was obviously shorter in patients with high SOX9 gene expression than in those with low expression (P ＜0.01).The noumethylation rate ofSOX9 gene promoter region in glioma tissues were 11.1％ in grade Ⅰ,33.3％ in grade Ⅱ,53.8％ in grade Ⅲ,71.9％ in grade Ⅳ respectively.There was significant difference between high-grade (Ⅲ-Ⅳ) group and low-grade group (Ⅰ-Ⅱ) (P ＜ 0.01).Conclusions The expression ofSOX9 gene upregulate and methylation of SOX9 gene in the promoter region decrease in human glioma.The patient's survival time is closely correlated with expression level ofSOX9 gene.
Keywords：gliomasSOX9 genemethylationepigenetics
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 417-420 )