Modeling of the facial nerve trunk local demyelination in SD rats
Yang Min
Tang Yinda
Ying Tingting
Zhu Jin
Li Shiting
Abstract：Objective By simulating the pathology of facial nerve injury for hemifacial spasm, to establish the models of the facial nerve trunk (FNT) local demyelination in SD rats. Methods The local quantitative injury was performed in the intracranial segment of the FNT in SD rats by using quantitative injury tweezers for establishing the animal model of the FNT local demyelination. The function of the facial nerve was evaluated by behavior and neuro-electrophysiological monitoring, and the never injury severity and demyelination severity were identified by pathological examination. Results When using 50 g quantitative injury force, the percentage of degenerated nerve fibers in injury side was about 38.6%, and the latency of F wave in the injury side was more significantly delayed than that in the normal side. Facial nerve was found obvious pathological change of demyelination, while there were no manifestations of facial nerve paralysis. The facial nerve fiber swelling, mild demyelination, and exfoliative myelin sheath after Wallerian degeneration were observed under electron microscope. Conclusions The model of FNT local demyelination in rats can be successfully established by using 50 g quantitative injury force. This animal model is stable, reliable and of good repeatability, which can accurately reflect the characteristics of hemifacial spasm in terms of manifestations, histopathology and electrophysiology.
Keywords：facial nerve diseaseshemifacial spasmnerve demyelinationanimal modelrats
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 131-134 )